It is known that an early church was in existence here by 1074. The original church of St Mary’s, Ardley, was probably built at around the same time as St Mary’s, Weston-on-the-Green, nearby. Both have a similar history, being part of the demesne of Robert d’Oilly of Oxford, granted to the church of St George in Oxford Castle, and later to Oseney Abbey.
The church now belongs to the Cherwell Valley Benefice, comprising Ardley, Fritwell, Lower Heyford, Upper Heyford, Somerton and Souldern.